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. Author manuscript; available in PMC: 2015 Mar 10.
Published in final edited form as: Exp Cell Res. 2014 Jan 23;322(1):39–50. doi: 10.1016/j.yexcr.2014.01.013

Figure 6. Effect of expressing Nrf2 in Nrf2−/− cells on copper-inducible transcription.

Figure 6

Nrf2−/− cells were transfected with the Nrf2 expression plasmid, pEF/Nrf2 and pARE4Luc, and then exposed to 300 μM copper for 24 h. The luciferase activity was then measured and normalized to the amount of β-galactosidase enzyme activity. Data are expressed as mean ± standard mean error. “*” indicates significantly different from cells exposed to copper only, by ANOVA, p < 0.05, n = 3 observations.