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. 2013 Dec 18;5(2):375–385. doi: 10.18632/oncotarget.1568

Figure 1. Cdk9 knock-down in MEC-1 cells and primary CLL cells.

Figure 1

In order to establish whether cdk9 is a valid therapeutic target, lentivirus containing cdk9 shRNA was produced. In the first instance (A) MEC-1 cells were infected with virus and following puromycin selection cells were cultured for 48h and cell viability assessed. (B) MEC-1 cells transfected with cdk9 shRNA showed significantly increased spontaneous apoptosis when compared with empty vector and scrambled sequence controls. (C) Next primary CLL cells were subjected to the same lentiviral infection protocol. Cdk9 was knocked down to approximately 50% of the empty vector control levels in these cells and this resulted in (D) a significant increase in spontaneous apoptosis. *P < 0.05, **P < 0.0001.