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. 2013 Dec 28;5(2):386–402. doi: 10.18632/oncotarget.1566

Figure 1. Validation of transgene expression in cultured 293-HEK and WiT49 cells.

Figure 1

(A) Transfection of wild-type CITED1 (C1-wt) in 293-HEK cells shows predominantly cytosolic immunofluorescent (IF) detection. (B) Transfection of CITED1ΔNES (C1-NES) shows enriched nuclear IF detection. (C) WiT49 cells transfected with empty vector control plasmid show rare and weak cytosolic CITED1 IF detection. (D) Wild-type CITED1 overexpression shows predominantly cytosolic IF detection. (E, F) CITED1ΔNES transfection into WiT49 cells shows robust nuclear and weak cytosolic IF detection. (G) Western blot for the FLAG epitope in 293-HEK and WiT49 cells transfected with experimental transgenes (p, passage number). (H) Western blot for FLAG-tagged CITED1 in WiT49 cells and two Wilms tumor specimens (VUWT1 and 2). (I) Direct sequencing confirms L165A and L167A mutations in the nuclear export signal (nuclear-preference) of the CITED1ΔNES plasmid.