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. 2014 Jan 12;5(2):451–461. doi: 10.18632/oncotarget.1296

Figure 4. A role for the GATA2 gene in focal adhesion disassembly.

Figure 4

A, following treatment with siGATA2, the smooth edge spindle-like LNCaP cells became flat and developed visible focal contacts. B, siGATA2-treated cells showed a clear focal adhesion immunostaining pattern in cells. Cells were immunostained with anti-vinculin-TRITC (red), anti-GATA2-FITC (green), and DAPI (blue). C, treatment with siGATA2 led to a failure in the disassembly of focal adhesions. Serum-starved siControl and siGATA2-treated LNCaP cells were incubated with 10 μM nocodazole for 4 hours. Cells at the indicated times after nocodazole washout were fixed and immunostained with anti-vinculin. Images were taken at 63x magnification.