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. 2014 Feb 27;2(3):253–261. doi: 10.1016/j.stemcr.2014.01.012

Figure 2.

Figure 2

OySyNyK-Induced iPSCs Exhibit Complete Pluripotency

(A–C) Hematoxylin and eosin-stained sections of teratomas derived from OySyNyK-induced iPSCs at 8 weeks after transplantation into immunodeficient mice. Representative images show tissues from all three germ layers: endoderm (A), mesoderm (B), and ectoderm (C). Teratomas were obtained from two iPSC lines. Scale bar, 200 μm.

(D and E) Images showing 8-week-old chimeric mice as indicated by an asterisk (∗). The agouti coat color was used to indicate the iPSC contribution (D). An OySyNyK-iPSC line was microinjected into blastocysts of ICR mice. The Agouti coat color of four offspring and their chimeric mother as indicated were used to confirm the germline competence of OySyNyK-iPSCs (E).

(F) Images showing dynamic changes of the GFP expression pattern among OSNK- and OySyNyK-transduced Oct4-GFP MEFs at different time points.

(G) DNA methylation patterns at the proximal promoter of the Oct4 or Nanog gene analyzed by bisulfite sequencing. Black circles indicate methylated CpG and open circles indicate unmethylated CpG.