Figure 4.

SOX2-YAP^TAD and Its Interaction with TET1/2 Are Critical for Rapid iPSC Induction by OySyNyK Factors

(A–C) Anti-SOX2 (A), anti-NANOG (B), and anti-OCT4 (C) antibodies were used for immunoprecipitation from ESC lysates. Anti-TET1 and anti-TET2 antibodies were then used to perform western blotting to detect whether TET1 or TET2 was present in the SOX2 and NANOG precipitants.

(D) Statistical summary showing that absence of SOX2-YAP^TAD greatly reduced the efficiency of iPSC generation. OySyNyK-induced GFP-positive colonies were counted at day 7; the other combinations were counted at day 12. ^∗∗∗p < 0.001 versus control groups.

(E) Statistical summary showing that SOX2-YAP^TAD plays critical role in the rapid iPSC induction by OySyNyK factors. OySyNyK-induced GFP-positive colonies were counted at day 7; the other combinations were counted at day 12. ^∗∗∗p < 0.001 versus control groups.

(F) Chromatin immunoprecipitation assays showing the enrichment of native SOX2 and the modified SOX2 at the promoters of Nanog, Oct4, and Tet1 from OSNK-induced and OySyNyK-induced MEFs, respectively, at day 1. ^∗∗∗p < 0.001 versus control groups.

Data from (D)–(F) are representative of at least three independent experiments (mean and SD of triplicate assays). Groups were compared using the Student’s t test. N.S, not significant; ^∗∗∗p < 0.001 versus control groups.