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. 2014 Mar 6;2(3):366–381. doi: 10.1016/j.stemcr.2014.01.014

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Genomic Stability and Pluripotency Are Maintained during Continuous Single-Cell and FF Culture

(A) Cytogenetic analysis on 20–40 G-banded metaphase cells from various hiPSC clones maintained in FF and single-cell culture.

(B) Flow cytometry profile and cytogenetic analysis of long-term-passaged hiPSC clones in FF and single-cell enzymatic-passaged culture.

(C) Three to 4-day-directed differentiation of FTC017-c11. Scale bar, 200 μm.

(D) Embryoid body formation and differentiation. Immunocytochemistry was conducted 28 days post-differentiation: ectoderm, Tuj1; mesoderm, α smooth muscle actin (aSMA); and endoderm, AFP. Scale bars, 1,000 μm (bright-field images), 200 μm (TUJ1 images), and 500 μm (SMA and AFP images).

(E) Histological sections of teratoma derived from FTC007-c21 and FTC016-c25. Black arrows point to endoderm, white arrows point to ectoderm, and gray arrows point to mesoderm. Scale bar, 200 μm.