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. 2014 Mar 6;2(3):366–381. doi: 10.1016/j.stemcr.2014.01.014

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Relative Gene Expression Profile of Minimal Factor Episomal-Induced hiPSCs in FMM

(A and B) Heatmap results derived from a Fluidigm dynamic array depicting relative gene expression levels of pluripotency (A) and differentiation (B) genes of conventionally maintained hiPSC lines, RNA from conventionally maintained H1 hESCs, and episomal hiPSC lines derived using various gene combinations maintained in FMM. Relative gene expression for each line is noted within each box and color coded based on three expression levels summarized in the legend (lower right). All sets were normalized to the average expression of two housekeeping genes (GAPDH and HPRT1) and referenced to the median expression level of six control conventional lines (OSK hiPSCs and H1 hESCs on MEF) representing 1× value for each gene. See also Figure S5.