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. 2014 Feb 27;2(3):271–281. doi: 10.1016/j.stemcr.2014.01.011

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The Role of Notch Signaling Activation in the Differentiation of Human Pluripotent Embryonic Stem Cells

Undifferentiated human embryonic stem cells were plated on control or oriented Jagged-1 surfaces, and gene expression was analyzed via quantitative PCR (qPCR) up to 8 days after initial plating. Notch gene: HES1; pluripotency gene: OCT4; mesodermal gene: BRACHYURYT; endodermal gene: FOXA2; ectodermal gene: SOX1.

(A) hESCs plated on oriented Jagged-1 surfaces display increased expression of the Notch target gene, HES1, peaking 1 day after plating, indicating successful activation of the Notch pathway via oriented Jagged-1 surfaces.

(B–E) Activation of Notch signaling does not alter pluripotency, mesoderm, or endoderm gene expression compared to controls but does increase ectodermal gene expression. Results are representative of three independent experiments and are mean ± SD, n = 3; symbols denote p < 0.05 compared to controls.