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. 2014 Feb 27;2(3):295–310. doi: 10.1016/j.stemcr.2014.01.009

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Whole-Cell Patch-Clamp Recordings of hESC-Derived Hippocampal Granule Neurons at Differentiation Week 4

(A–D) The majority of the neurons patched (eight out of ten) showed Na⁺/K+ currents (A) and evoked action potentials (C) as well as spontaneous bursts of action potentials (B) and spontaneous postsynaptic currents (D).

(E and F) Quantification of the frequency and amplitude of spontaneous postsynaptic currents in hESC-derived hippocampal granule neurons at 4 weeks postdifferentiation.

All data are presented as mean ± SEM.