. 2013 Oct 29;27(2):51–58. doi: 10.1007/s13577-013-0080-9

Table 1.

Real-time polymerase chain reaction (RT-PCR) primer sequences for canine-neural-specific genes

Gene	Ref sequence	Primer sequence (5′-3′)	Annealing temperature (°C)	Size (bp)
NEFL	NC_006607	Forward GAAGAAGCTGCCAAGGAAGA	58	202
NEFL	NC_006607	Reverse GTTGACCTGATTTCGGGAGA	58	202
GFAP	NC_006591	Forward ACCTGGCCAGTTATCGACAG	58	206
GFAP	NC_006591	Reverse TCTTAGGGCTGCTGTGAGGT	58	206
NSE	NC_006609	Forward GAGAACAGTGAAGCCTTGGA	60	298
NSE	NC_006609	Reverse ACCAATCTGGTTGACCTTGA	60	298
TUBB3	NC_006587	Forward GCACACTGCTCATCAACAAG	60	227
TUBB3	NC_006587	Reverse TCTTGCTCTCCTTCATGGAC	60	227
B2M	NC_006612	Forward TCTACATTGGGCACTGTGTCAC	58	136
B2M	NC_006612	Reverse TGAAGAGTTCAGGTCTGACCAAG	58	136