Table 1.

Extraction and detection methods for S. endobioticum

Method	Citation
Separation of sporangia from soil particles based on different specific gravity in chloroform	Glynne (1926), Pratt (1974, 1976a), Hampson and Thompson (1977), Hampson and Robertson (1995)
Wet sieving using electromagnetic shaker aided by chloroform floatation	Hampson and Coombes (1996)
Soil extraction with the non ionic detergent Triton X100	Laidlaw (1985)
Substitution of chloroform by dibromomethane and oil, use of fluoric acid for sand removal	Nelson and Olsen (1964)
Extraction in water and sedimentation using sodium sulfate	Marcus (1969), Mygind (1954; 1961)
Extraction with potassium iodide from air dried soil after sieving	Putnam and Sindermann (1994)
Density gradients with potassium/sodium salt on air dried soil after sieving	Zeyla and Melnik (1998)
Extraction with chloroform, calcium chloride and zinc sulfate	van Leeuwen et al. (2005)
Zonal centrifugation	Wander et al. (2007)
Microscopic examination	National Diagnostic Protocol (2011)
Polymerase chain reaction (PCR) using species-specific primers	Niepold and Stachewicz (2004), Van den Boogert et al. (2005), van Gent-Pelzer et al. (2010)
Microarray-based hybridization	Abdullahi et al. (2005)