Table 3.
Stable candidate QTL associated with seed quality traits identified at both Manitoba (MB) and Saskatchewan (SK) locations
| Trait | Contig–scaffold-marker | Allele size (bp) | LG | Position | −Log10 (P) | QTL | Size (cM) | R 2 (%) | LD (r 2)a | Effect | IPCA1 | ASV |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| OIL | c31-s67_Lu181 | 270 | 9 | 31.34 | 3.73 | QOil-LG9.1 | 1.20 | 7.56 | 0.27 | 1.33** | −1.062 | 2.38 |
| STE | c175-s1216_Lu146 | 354 | 7 | 23.95 | 6.23 | QSte-LG7.1 | 0.01 | 19.68 | 0.71 | 1.67** | −0.241 | 0.40 |
| LIO | c729-s156_Lu3262 | 217 | 3 | 55.74 | 5.10 | QLio-LG3.1 | 8.70 | 6.60 | 0.24 | 1.09** | −0.701 | 1.67 |
| c30-s11_Lu164 | 211 | 5 | 57.89 | 3.52 | QLio-LG5.2 | 0.90 | 3.31 | 0.11 | 0.43* | 1.239 | 2.16 | |
| c306-s98_Lu765Bb | Null | 12 | 75.12 | 8.40 | QLio-LG12.3 b | 3.20 | 13.6 | 0.93 | 0.90* | 0.489 | 0.78 | |
| LIN | c729-s156_Lu3262 | 217 | 3 | 55.74 | 5.57 | QLin-LG3.1 | 8.70 | 5.33 | 0.24 | 1.24** | 0.501 | 1.09 |
| c202-s39_Lu41 | 323 | 5 | 57.36 | 5.99 | QLin-LG5.2 | 0.90 | 9.31 | 0.11 | 1.79** | 0.302 | 1.04 | |
| c306-s98_Lu765Bb | Null | 12 | 75.12 | 4.86 | QLin-LG12.3 b | 3.20 | 6.06 | 0.93 | 0.63* | −0.890 | 1.16 | |
| IOD | c46-s505_Lu2102 | 241 | 8 | 72.74 | 4.23 | QIod-LG8.1 | 1.60 | 9.35 | 0.22 | 9.31** | 0.807 | 1.05 |
Significance of the allelic effects tested by Kruskal–Wallis non-parametric test * P < 0.01; ** P < 0.001
aStrength of the physical linkage between markers ranges from 0 (no linkage or no correlation between alleles at different loci) to 1 (total linkage or perfect correlation between alleles at different loci)
bCandidate QTL previously reported (Cloutier et al. 2011)