Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jun 11;9:672. doi: 10.1038/msb.2013.26

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013, EMBO and Macmillan Publishers Limited

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/.

PMC Copyright notice

Construction and validation of EGFP–FLAG tagged HDACs 1–11. (Left) HDAC 1–11 tagged with EGFP (green)–FLAG (red) at their C terminus. Boundaries of the deacetylase (yellow), MEF2 binding (brown), nuclear localization signal NLS (blue) and nuclear export signal NES (orange) regions are indicated. (Center) Deacetylase activity of HDACs isolated from CEM T cells measured using the Fluor-de-Lys assay (n=3, AFU±s.d.), as compared to EGFP–FLAG controls. (Right) Localization of EGFP–FLAG tagged HDACs in CEM T-cell lines using anti-GFP antibody (green); DNA is indicated by DAPI (blue); × 63 oil immersion lens; scale bar, 5 μm.