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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2013 Apr 11;26(4):571–579. doi: 10.1111/pcmr.12091

Figure 4. Melanoma cells express membrane-bound TNF-α.

Figure 4

A) Melanoma cells express TNF-α gene message. Expression of TNF-α message was assessed by real time PCR, relative to the housekeeping gene GADPH. Representative data from one of three similar experiments are shown. B) Melanoma cells express mTNF. Open histograms, unstained; shaded histograms, stained with specific mAb. Staining with isotype control was not different than unstained (not shown). Representative data from one of three similar experiments; comparable data were obtained for all cell lines listed in Panel A.