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. 2014 Feb 15;4(5):460–474. doi: 10.7150/thno.8077

Figure 1.

Figure 1

Figure 1

A. Microscopic images of MDA-MB-231 stable cells expressing HSV1-sr39TK, NTR and HSV1-sr39TK-NTR fusion 3 h after exposure to CytoCy5S, a quenched fluorescent substrate of NTR. Left panel shows bright field images and right panel shows the fluorescent microscopic images of stable cells entrapping reduced CytoCy5S dye. B. FACS histogram overlay of MDA-MB-231 HSV1-sr39TK, NTR and HSV1-sr39TK-NTR stable cells treated with CytoCy5S dye. C. Graph shows 3H-PCV uptake of MDA-MB-231 stable cells expressing HSV-sr39TK, NTR and HSV-sr39TK-NTR fusion proteins. D. Quantitative RT-PCR analysis of MDA-MB-231 stable cells expressing HSV1-sr39TK, NTR and HSV1-sr39TK-NTR fusion shows nearly equal level of mRNA expression for stably transfected HSV1-sr39TK, NTR, HSV1-sr39TK-NTR fusion. E. Immunoblot analysis shows the expression of HSV1-sr39TK and HSV1-sr39TK-NTR fusion protein stained with TK specific antibody.