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. 2014 Mar 13;63(4):1366–1380. doi: 10.2337/db13-1412

Figure 3.

Figure 3

Effect of Rap1b G12V on renal morphology, ECM expression, oxidative stress, and apoptosis in rats kidneys with STZ-induced diabetes. A: Kidney sections were stained with PAS (top panels) and anti–Col-1 (middle panels) and -FN antibodies (bottom panels) (magnification ×400). B and C: Semiquantification of IHC staining of Col-1 and FN. D: Using Western blot analysis, Rap1b G12V inhibited the expression of Col-1 and FN in rats with STZ-induced diabetes. E and F: Quantification of average band density calculated from different Western blots. G: Increased oxidative stress and apoptosis was seen in tubular cells of diabetic rat kidneys, as assessed by DHE (top panels) and TUNEL-F (middle panels) or -H (bottom panels) staining, while the effect was reduced by transfection of Rap1b G12V plasmid into the kidney. H–J: Quantification of tissues stained with DHE, TUNEL-F, and TUNEL-H procedures. Values are means ± SE. *P < 0.01 versus control; #P < 0.01 versus STZ. N = 6. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.