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. 2014 Mar 13;63(4):1394–1409. doi: 10.2337/db13-0334

Figure 6.

Figure 6

4548-G05 displays hypoglycemic activity in diabetic animal models. A: 4548-G05 activates insulin signaling in db/db mice. Eight-week-old mice were starved for 12 h and then 4548-G05 (5 mg/kg) was orally administered. Cell lysates were prepared from liver, muscle, and fat tissues that were collected at different time intervals and analyzed by immunoprecipitation and immunoblotting. B: 4548-G05 reduces blood glucose levels in fed db/db mice. Vehicle (0.5% methylcellulose) or 4548-G05 (5 mg/kg) was orally administered to the animals. Blood glucose was monitored before and after dosing at 1-h intervals. *P < 0.05 vs. vehicle; two-way ANOVA (n = 6). C: 4548-G05 reduces blood glucose level in fasted db/db mice. The animals were starved for 12 h and then vehicle (0.5% methylcellulose) or 4548-G05 (5 mg/kg) was orally administered. Blood glucose was monitored before and after dosing at 1-h intervals (n = 5). ***P < 0.001 vs. vehicle; two-way ANOVA. D: 4548-G05 improves the glucose tolerance in db/db mice. The animals were starved for 12 h and then vehicle (0.5% methylcellulose) or 4548-G05 (5 mg/kg) was orally administered 1 h before a bolus injection of glucose (0.3 mg/kg). Blood glucose was measured at the indicated time intervals (n = 5). E: Area under the curve of the glucose tolerance test shown in D. *P < 0.05, Student t test (n = 5). F: Hypoglycemic activity of 4548-G05 in mice with STZ-induced T1DM. C57BL/6 mice (8 weeks old) were divided into two groups 7 days after a single intraperitoneal injection of STZ (150 mg/kg). The animals then were starved for 12 h and vehicle (0.5% methylcellulose) or 4548-G05 (5 mg/kg) was orally administered. Blood glucose was measured at the indicated time intervals. ***P < 0.001 vs. vehicle; two-way ANOVA (n = 5). G: Chronic treatment with 4548-G05 given to db/db mice improves the hyperglycemia. Vehicle (0.5% methylcellulose) or 4548-G05 (5 mg/kg) was administrated orally once a day for 10 consecutive days to 8-week-old db/db mice. Blood glucose of the fed animals then was measured at various time intervals as indicated. *P < 0.05, two-way ANOVA (n = 5).