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. Author manuscript; available in PMC: 2014 Mar 25.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7982–7989. doi: 10.4049/jimmunol.0803073

FIGURE 4.

FIGURE 4

THP-1 cells stably expressing siRNA against MEFV (pyrin) show decrease in IL-1β release, followed by F. novicida infection. THP-1 cells stably expressing siPyrin show decreased MEFV (pyrin) mRNA (A) and intracellular pyrin (D) levels after infection with Francisella, as compared with control siRNA (siCtr) group. These stable THP-1 show a similar IL-1β (B) and IL-8 (C) mRNA synthesis after infection with F. novicida. However, following F. novicida infection, THP-1 cells with decreased levels of pyrin show decrease in mature IL-1β release (p = 0.003) (E). In contrast, in response to Francisella infection, inflammasome-independent IL-8 release is not affected by pyrin knockdown (F). To note, stable THP-1 not infected with F. novicida do not release IL-1β (<0.005 ng/ml) and IL-8 (<0.1 ng/ml), and therefore, are not included in the figure. Experiments represent n = 6 of independent experiments done in duplicates and triplicates.