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. Author manuscript; available in PMC: 2014 Mar 25.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7982–7989. doi: 10.4049/jimmunol.0803073

FIGURE 5.

FIGURE 5

M-CSF induces pyrin expression and ability to respond to Francisella. Francisella infection activates an inflammasome only in pyrin-expressing MDM, as indicated by an active caspase-1 (p20) and mature IL-1β release into cell culture medium. MDM matured in the absence or constant presence of M-CSF (20 ng/ml) were infected with F. novicida for 15 h. Cell culture medium was collected and assayed for IL-1β and caspase-1 (A). Cells were lysed and immunoblotted for pro-IL-1β and pyrin (B). Two representative donors of six are shown.