Abstract
Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.
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Selected References
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- Crofts A. R. Amine uncoupling of energy transfer in chloroplasts. I. Relation to ammonium ion uptake. J Biol Chem. 1967 Jul 25;242(14):3352–3359. [PubMed] [Google Scholar]
- Filner P. Regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1966 May 5;118(2):299–310. doi: 10.1016/s0926-6593(66)80038-3. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Can J Biochem. 1966 Jun;44(6):791–799. doi: 10.1139/o66-097. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L., Eveleigh D. E. Culture methods and detection of glucanases in suspension cultures of wheat and barley. Can J Biochem. 1968 May;46(5):417–421. doi: 10.1139/o68-063. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
- Gamborg O. L. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 1970 Apr;45(4):372–375. doi: 10.1104/pp.45.4.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katunuma N., Okada M., Nishii Y. Regulation of the urea cycle and TCA cycle by ammonia. Adv Enzyme Regul. 1966;4:317–336. doi: 10.1016/0065-2571(66)90025-2. [DOI] [PubMed] [Google Scholar]
- Woolfolk C. A., Shapiro B., Stadtman E. R. Regulation of glutamine synthetase. I. Purification and properties of glutamine synthetase from Escherichia coli. Arch Biochem Biophys. 1966 Sep 26;116(1):177–192. doi: 10.1016/0003-9861(66)90026-9. [DOI] [PubMed] [Google Scholar]