Table 5.
Global miRNA profiling of MIN6 cells cultured with human native and oxidized LDL with or without HDL. We compared by microarray analysis the expression of 350 miRNAs in MIN6 cells that were incubated with 2 mmol/L of human native (Na LDL) or oxidized LDL (oxLDL) cholesterol plus or minus 1 mmol/L of HDL for 72 hrs.
Microarray | Name | NaLDL | oxLDL | Change (log2) | Expression change | oxLDL | oxLDL + HDL | Change (log2) |
---|---|---|---|---|---|---|---|---|
NaLDL versus oxLDL | mmu-miR-9 | 1 622.36 | 739.3 | −1.16 | Down | 2 417.08 | 3 407.71 | 0.46 |
mmu-miR-21 | 6 073.84 | 15 447.81 | 1.37 | Up | 23 042.48 | 11 193.24 | −1.01 | |
mmu-miR-98 | 7 909.01 | 2 817.75 | −1.5 | Down | 12 432.59 | 16 691.57 | 0.42 | |
mmu-miR-192 | 222.59 | 514.66 | 1.02 | Up | 1 076.26 | 732.38 | −0.51 | |
mmu-miR-325 | 1 018.38 | 462.41 | −1.16 | Down | 1 757.68 | 2 642.59 | 0.65 | |
mmu-miR-342-3p | 2 181.11 | 1 326.71 | −0.73 | Down | 2 483.59 | 4 337.97 | 0.79 | |
mmu-miR-346 | 904.18 | 505.43 | −0.85 | Down | 485.7 | 770.91 | 0.64 | |
mmu-miR-374 | 5 082.44 | 1 887.82 | −1.43 | Down | 5 022.55 | 7 143.28 | 0.54 | |
Mmu-miR-708 | 366.85 | 712.44 | 0.94 | up | 825.11 | 402.52 | −1.06 | |
mmu-miR-801 | 488.33 | 915.52 | 0.85 | up | 521.23 | 216.79 | −1.29 |