Figure 2.

Growth of fusion strains at 4°C and 37°C. Strains of L. monocytogenes EGD-e wild-type, ΔsigB, and ΔrsbV containing P_lmo2230::egfp fusion were grown at (a) 4°C and at (b) 37°C in BHI. Time points when samples of cells were taken for flow cytometry analyses were marked with a dashed line. The charts show representative results of three biological replicates carried out in duplicate in each replication. (c) Specific growth rates of fusion strains grown at 4°C and 37°C were calculated for exponentially growing cultures.