Table 1.

Plasmids and strains used in this study.

Plasmid	Abbreviated name	Source or reference	Collection number
Champion pET101 directional TOPO expression  vector containing topoisomerase for efficient and simple cloning	pET101D	Invitrogen	N/A
Champion pET101 directional TOPO expression  vector with wild type rsbW with N-terminal His6-tag	pEC02	This study	N/A
Champion pET101 directional TOPO expression  vector with wild type sigB with N-terminal His6-tag	pEC03	This study	N/A
Strains
One shot TOP10 chemically competent E. coli  F−   mcrA Δ(mmr-hsdRMA-mcrBC) Φ80lacZΔM15  ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK  rpsL (StrR) endA1 nupG	E. coli TOP10	Invitrogen	N/A
BL21 Star (DE3) one shot chemically Competent E.  coli  F−   impT hsdSB (rB −mB −) gal  dcm  rne131 (DE3)	E. coli BL21 (DE3)	Invitrogen	N/A
BL21 Star (DE3) E. coli/pEC02	E. coli/rsbW-his6	This study	COB275
BL21 Star (DE3) E. coli/pEC03	E. coli/sigB-his6	This study	COB276
L. monocytogenes EGD-e	wt	K. Boor	COB261
L. monocytogenes EGD-e Δ sigB	ΔsigB	K. Boor	COB262
L. monocytogenes EGD-e Δ rsbV	ΔrsbV	Utratna et al. 2012 [24]	COB411
L. monocytogenes EGD-e::pKSV7-Plmo2230::egfp	wt-egfp	Utratna et al. 2012 [24]	COB491
L. monocytogenes EGD-e ΔsigB::pKSV7-Plmo2230::egfp	ΔsigB-egfp	Utratna et al. 2012 [24]	COB495
L. monocytogenes EGD-e ΔrsbV::pKSV7-Plmo2230::egfp	ΔrsbV-egfp	Utratna et al. 2012 [24]	COB501