Champion pET101 directional TOPO expression vector containing topoisomerase for efficient and simple cloning |
pET101D |
Invitrogen |
N/A |
Champion pET101 directional TOPO expression vector with wild type rsbW with N-terminal His6-tag |
pEC02 |
This study |
N/A |
Champion pET101 directional TOPO expression vector with wild type sigB with N-terminal His6-tag |
pEC03 |
This study |
N/A |
|
Strains |
One shot TOP10 chemically competent E. coli F−
mcrA Δ(mmr-hsdRMA-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG |
E. coli TOP10 |
Invitrogen |
N/A |
BL21 Star (DE3) one shot chemically Competent E. coli F−
impT hsdSB (rB
−mB
−) gal dcm rne131 (DE3) |
E. coli BL21 (DE3) |
Invitrogen |
N/A |
BL21 Star (DE3) E. coli/pEC02 |
E. coli/rsbW-his6
|
This study |
COB275 |
BL21 Star (DE3) E. coli/pEC03 |
E. coli/sigB-his6
|
This study |
COB276 |
L. monocytogenes EGD-e |
wt |
K. Boor |
COB261 |
L. monocytogenes EGD-e Δ sigB
|
ΔsigB
|
K. Boor |
COB262 |
L. monocytogenes EGD-e Δ rsbV
|
ΔrsbV
|
Utratna et al. 2012 [24] |
COB411 |
L. monocytogenes EGD-e::pKSV7-Plmo2230::egfp
|
wt-egfp
|
Utratna et al. 2012 [24] |
COB491 |
L. monocytogenes EGD-e ΔsigB::pKSV7-Plmo2230::egfp
|
ΔsigB-egfp
|
Utratna et al. 2012 [24] |
COB495 |
L. monocytogenes EGD-e ΔrsbV::pKSV7-Plmo2230::egfp
|
ΔrsbV-egfp
|
Utratna et al. 2012 [24] |
COB501 |