Table 1.
Genes whose expression is significantly lower in Lrp5−/− bone compared to wild-type bone, and higher inwild-type bone treated with Scl-AbIII compared to vehicle alone. Differences in expression between mice lacking Lrp5 (Lrp5−/−) and mice with functioning Lrp5 (Lrp5+/+ or Lrp5A214V/+) are reported as fold changes, along with their corresponding P values, as are the differences in expression between mice that received Scl-AbIII and those that received vehicle [phosphate-buffered saline (PBS)] alone.
| Gene | Protein product | Lrp5+/+
|
Lrp5−/−/Lrp5+/+
|
Lrp5−/−/Lrp5A214V/+
|
Scl-Ablll/PBS |
|||
|---|---|---|---|---|---|---|---|---|
| RPKM* | Fold change | P | Fold change | P | Fold change | P | ||
| Cpz | Carboxypeptidase Z | 11.55 | 0.16 | 1.08 × 10−24 | 0.18 | 7.29 × 10−17 | 4.77 | 2.72 × 10−13 |
| Slc13a5 | Solute carrier family 13 (sodium-dependent citrate transporter), member 5 |
64.14 | 0.17 | 9.76 × 10−24 | 0.18 | 1.81 × 10−17 | 4.77 | 7.90 × 10−14 |
| Col1a1 | Collagen, type 1, α1 | 1245.3 | 0.23 | 1.48 × 10−17 | 0.23 | 1.43 × 10−13 | 5.4 | 8.67 × 10−16 |
| Prss35 | Protease, serine, 35 | 34.62 | 0.24 | 1.16 × 10−15 | 0.41 | 4.05 × 10−5 | 4.57 | 3.22 × 10−13 |
| Col11a2 | Collagen, type XI, α2 | 94.39 | 0.25 | 2.14 × 10−15 | 0.29 | 8.05 × 10−10 | 4.08 | 1.75 × 10−11 |
| Bglap2 | Bone γ-carboxyglutamate protein 2 | 338.97 | 0.31 | 3.94 × 10−11 | 0.39 | 4.64 × 10−6 | 6.67 | 8.91 × 10−21 |
| Col1a2 | Collagen, type 1, α2 | 981.29 | 0.35 | 3.18 × 10−9 | 0.35 | 8.03 × 10−7 | 4.42 | 2.47 × 10−12 |
| Bglap | Bone γ-carboxyglutamate protein | 261.73 | 0.38 | 8.22 × 10−8 | 0.37 | 4.32 × 10−6 | 6.23 | 1.90 × 10−19 |
| Cdo1 | Cysteine dioxygenase 1, cytosolic | 63.39 | 0.42 | 4.87 × 10−6 | 0.32 | 2.65 × 10−7 | 3.42 | 3.92 × 10−9 |
| Cgref1 | Cell growth regulator with EF hand domain 1 | 25.61 | 0.48 | 2.05 × 10−4 | 0.43 | 1.81 × 10−4 | 4.01 | 7.19 × 10−11 |
| Kcnk1 | Potassium channel, subfamily K, member 1 | 10.08 | 0.48 | 3.37 × 10− 4 | 0.42 | 3.28 × 10−4 | 5.06 | 3.63 × 10−14 |
| Pla2g5 | Phospholipase A2, group V | 7.67 | 0.49 | 6.80 × 10−4 | 0.38 | 5.03 × 10−5 | 2.85 | 1.81 × 10−6 |
*
RPKM value is the reads per kilobase coding sequence per million reads for the transcript in wild-type bone. Higher RPKM values indicate higher levels of mRNA expression.