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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2013 Nov 12;20(2):192–201. doi: 10.1016/j.bbmt.2013.11.007

Figure 4.

Figure 4

Apoptosis of alloreactive CD4+ T cells induced by IL-12hi RAPA-DCs is dependent on IL-12 expression. Wild-type B6 or B6 IL-12p40−/− myeloid DCs (mDC) were generated and then used in 5-day MLRs to stimulate BALB/c CD4+ T cells. Plots are representative of 3 independent experiments performed. (A) LPS-exposed IL-12p40−/− RAPA-DCs induce decreased apoptosis (Annexin V+7-AAD+) of alloreactive CD4+ T cells compared with wild-type (16.9% ± 8.9% versus 20.8% ± 10.0%; P < .05). Blocking IFN-γ further decreased apoptosis stimulated by LPS-exposed IL-12p40−/− RAPA-DCs (12.0% ± 6.1% versus 16.9% ± 8.9%, P < .05). (B) Average fold increase in CD4+ Annexin V+ 7-AAD+ cells relative to wild-type CTR-DCs on day 5 of MLRs. Error bars indicate mean + 1 SD. n = 3. *P < .05.