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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2013 Nov 12;20(2):192–201. doi: 10.1016/j.bbmt.2013.11.007

Figure 6.

Figure 6

Autologous IL-12hi RAPA-DC demonstrate low costimulatory molecule expression and inhibit acute GVHD. BALB/c CTR- and RAPA-DCs were generated and then analyzed for cell surface marker expression using flow cytometry. (A) Compared with equivalent CTR-DC groups, unstimulated RAPA- and LPS-exposed RAPA-DCs showed decreased surface expression (MFI) of IAd, CD86, and CD80. (B) BALB/c LPS-stimulated RAPA-DCs demonstrate increased IL-12 expression relative to LPS-stimulated CTR-DCs (CD11c+ IL-12hi population 67.3% versus 16.3%). (C) Lethally irradiated BALB/c recipients were given 5 × 106 B6 bone marrow cells plus 1 × 106 purified B6 T cells alone (GVHD control) or with 1 × 106 BALB/c DCs (CTR-DC ± LPS or RAPA-DC ± LPS). Irradiated BALB/c mice injected with syngeneic BALB/c bone marrow (syngeneic) are also indicated. Although mice receiving CTR-DCs had survival comparable with the control GVHD group (without DCs) (median survival in days: GVHD, 17.5 days; CTR, 8 days; CTR-LPS, 11 days; all P = NS), IL-12hi RAPA-DCs significantly and markedly prolonged survival from GVHD (median survival in days: 38 days, P =.007).