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. Author manuscript; available in PMC: 2014 Mar 25.
Published in final edited form as: Sci Signal. 2013 Aug 20;6(289):ra70. doi: 10.1126/scisignal.2004145

Fig. 5. The FACT complex is an evolutionarily conserved regulator of nucleolar size.

Fig. 5

(A) Small nucleolus phenotype in S. cerevisiae FACT complex mutants. Images are representative of five independent biological replicates. (B) Examples of nucleolar size phenotypes in D. melanogaster third instar larval salivary glands upon knockdown of the indicated genes using transgenic RNAi. Images are representative of five independent biological replicates. (C) Quantification of the indicated nucleolar size phenotypes is shown in (B). Bars represent means ± SEM. ***P < 0.001; **P < 0.01; *P < 0.05. (D) Loss of germline cells upon knockdown of spt16 using two independent, non-overlapping RNAi constructs. Images are representative of four independent biological replicates. (E) Suppression of neural stem cell tumor growth in D. melanogaster (assayed by tumor-specific luciferase expression) upon targeting genes that result in smaller nucleoli upon transgenic RNAi. Bars represent the means ± SEM of four independent biological replicates. ***P < 0.001.