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. 2013 Dec 6;9(3):673–682. doi: 10.1021/cb400618m

Figure 1.

Figure 1

The co-receptor complex is specific for auxin. (a) Sensorgram showing the binding of TIR1 to degron peptide IAA7 on the chip in the absence (blue) and presence of IAA (500 μM; gray). (b) As in panel a, but showing the absence of binding even to a mutagenized IAA7 peptide (IAAm7). (c) The assembly of the TIR1 co-receptor complex is not dependent on exogenous IP6. (d) The response to auxin is dose-dependent. Sensorgrams in panels c and d show ΔRU using channels 4-3 (binding to peptide IAA7 minus peptide IAAm7). (e) The co-receptor complex remains stable in the presence of IAA. The dissociation of the complex is markedly reduced with IAA in the wash buffer (dark blue) compared to dissociation without auxin in the wash buffer (mid and light blue, repeats). Calculated kd with IAA = 1.09 × 10–3 s–1; kd without IAA = 5.58 × 10–3 s–1; X2 = 1.07.