Fig. 8.

Amino acid positions in the holoBirA dimer interface at which amino acid substitutions are accompanied by a ≥1 kcal/mol change in the absolute value of the dimerization free energy. The color coding is identical with that used for the interface loops in Fig. 2a. The model was prepared in PyMOL [5] using Protein Data Bank file 2EWN as input. Please refer to the text for discussion of side chains K194 and D197.