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. 2014 Apr;20(4):474–482. doi: 10.1261/rna.041376.113

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© 2014 Gonçalves et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 1. — Effect of SRSF1 and SRSF3 on alternative splicing of Rac1b in NCM460 cells. (A) The normal colonocyte cell line NCM460 was cotransfected with a RAC1 minigene and the indicated T7-tagged splicing factors. The Western blot detection of T7-SRSF1 and T7-SRSF3 is shown 24 h after transfection in NCM460 with endogenous α-tubulin as loading control. qPCR quantification of the corresponding minigene-derived transcripts is shown in the lower panel. (B) NCM460 cells were transfected with the indicated siRNAs and analyzed after 48 h. A Western blot detection of endogenous levels of Rac1b is shown, together with those of SRSF1 and SRSF3, to document successful knockdown and of α-tubulin as loading control. The lower panel shows quantification of the corresponding splicing changes in endogenous Rac1 and Rac1b transcript levels by qPCR.