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. 2014 Apr;20(4):474–482. doi: 10.1261/rna.041376.113

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© 2014 Gonçalves et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 2. — Effect of shRNA-mediated depletion of selected protein kinases on endogenous Rac1b protein levels. NCM460 cells were transiently transfected with shRNAs against the indicated kinases, lysed after 48 h, and analyzed by Western blot for endogenous Rac1b protein and α-tubulin as loading control. Band intensities from the primary screen blot were determined by densitometry and plotted in the graph below to display fold changes in Rac1b protein expression compared to control cells and identify candidate kinases for subsequent analysis.