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. 2014 Apr;20(4):559–567. doi: 10.1261/rna.042267.113

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© 2014 Sundaram and Grant; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 1. — Regulation of C. albicans translation initiation by Gcn2. (A) The wild-type and gcn2 mutant strains were treated with the indicated concentrations of 3AT for 1 h. Immunoblots are shown probed with antibodies specific for phosphorylated eIF2α and translation elongation factor 1 (Tef1) as a loading control. (*) A nonspecific band recognized by anti-eIF2αP. (B) Polyribosome traces are shown for strains treated with the indicated concentrations of 3AT for 1 h. Peaks correspond to small ribosomal subunits (40S), large ribosomal subunits (60S), monosomes (80S), and increasing numbers of ribosomes bound to mRNAs (polysomes). (C) Protein synthesis was measured by pulse labeling with [³⁵S]-cysteine/methionine for 5 min. Data are shown for untreated cultures (100%) and following treatments with different concentrations of 3AT. (D) Strains were grown to stationary phase and diluted cultures (A₆₀₀ = 1.0, 0.1, and 0.01) spotted onto SCD agar plates containing the indicated concentrations of 3AT.