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. 2014 Apr;20(4):559–567. doi: 10.1261/rna.042267.113

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© 2014 Sundaram and Grant; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 4. — Regulation of GCN4 translation by uORFs. (A) The wild-type and gcn2 mutant strains containing a wild-type GCN4-Luc construct were treated with 15 mM 3AT for 3 h. The uORFs in GCN4 are indicated as boxes in the schematic. (B) The wild-type and gcn2 mutant strains containing a GCN4-Luc construct where uORF1–3 were mutated were treated with 15 mM 3AT for 1 h. Mutations in the uORFs of GCN4 are indicated as crosses in the schematic. (C) Quantitative RT-PCR analysis of GCN4-Luc mRNA levels relative to GAPDH mRNA levels. (D) Quantitative RT-PCR analysis of GCN4 mRNA levels relative to GAPDH mRNA levels. White-colored bars denote values obtained from nonstressed cells; black-colored bars, values obtained from cells treated with 15 mM 3AT for 3 h.