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Regulation of GCN4 expression depends on a leaky scanning mechanism at uORF3. Two different stem–loop structures (ΔG = −8.7 kcal and −41 kcal) were introduced into GCN4-Luc lacking uORFs (A) or containing uORFs (B) as indicated. Fold-induction is indicated in brackets. White-colored bars denote values obtained from nonstressed cells; black-colored bars, values obtained from cells treated with 15 mM 3AT for 3 h.
