Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr;20(4):568–579. doi: 10.1261/rna.043513.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Zhang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

FIGURE 3. — Copurification of AnaPnp with the His-tagged GFP-AnaRneC fusions expressed in Anabaena cells using Ni-NTA beads. (A) Schematic presentation of the His-tagged GFP and GFP-AnaRneC fusions expressed in Anabaena cells. (B) Total proteins from Anabaena cells expressing the His-tagged recombinant proteins were applied to the Ni-NTA columns for purification. Proteins eluted were analyzed by SDS-PAGE gels, followed by silver staining or immunodetection using polyclonal antibodies against AnaPnp. Lanes 1–3, samples from Anabaena cells expressing hGFP, hGFP-EC, and EC-GFPh, respectively.