NMR CSs |
Local structural propensities (poly-proline II, α-helix and β-strand populations) |
NMR PREs |
Detection of distances between regions distant in primary sequence (one containing a spin-label) |
NMR RDCs |
Local structural propensities |
Cooperativity of secondary structures |
Transient long-range interactions |
NMR spin relaxation (15N, 13C) |
Differential rigidity |
Local dynamic timescales and amplitudes |
NMR relaxation dispersion |
Characterization of weakly populated states using CSs/RDCs (see above). |
Conformational exchange on micro-millisecond timescales (folding/binding) |
Small angle scattering (SAXS/SANS) |
Pairwise distribution function of long-range distances |
FRET |
Long-range interactions |