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. 2013 Oct 29;42(Database issue):D326–D335. doi: 10.1093/nar/gkt960

Table 2.

The type of structural information obtained from the different types of experimental parameters used to calculate pE-DB ensembles

Experimental parameter Major conformational information for IDPs
NMR CSs Local structural propensities (poly-proline II, α-helix and β-strand populations)
NMR PREs Detection of distances between regions distant in primary sequence (one containing a spin-label)
NMR RDCs Local structural propensities
Cooperativity of secondary structures
Transient long-range interactions
NMR spin relaxation (15N, 13C) Differential rigidity
Local dynamic timescales and amplitudes
NMR relaxation dispersion Characterization of weakly populated states using CSs/RDCs (see above).
Conformational exchange on micro-millisecond timescales (folding/binding)
Small angle scattering (SAXS/SANS) Pairwise distribution function of long-range distances
FRET Long-range interactions