Fig. 3.
Semi-quantitative RT-PCR analysis of Tctra-2 mRNA levels. Primers F1 and R1 shown in Fig. 1 and cDNA copied from RNA isolated from staged embryos (A), larvae, pupae or adults (B) as well as ovary and testis (C) dissected from adults were used to amplify tra-2. Bottom panels in each Figure show rp49 amplified using the same cDNA samples. NHL, newly hatched first instar larvae.