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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Insect Biochem Mol Biol. 2013 Sep 19;43(12):1125–1132. doi: 10.1016/j.ibmb.2013.08.010

Fig. 4.

Fig. 4

Sex-specific splicing of Tctra (A & B) and Tcdsx (C & D) in insects injected with malE or Tctra-2 dsRNA. malE or Tctra-2 dsRNAs were injected into newly ecdysed pupa (A & C) or newly emerged adults (B & D). Total RNA was isolated on 6th day after injection of dsRNA and cDNAs were synthesized. RT-PCR was done to detect the splicing status of Tctra and Tcdsx using the primers reported previously (Shukla and Palli, 2012b). Tctra and Tcdsx isoforms were detected by resolving RT-PCR products on agarose gels. Bottom panels in each Figure show rp49 amplified using the same cDNA samples.