Figure 13. Ultrastructure of IL1 and IL2 neurons.
(A) 3D reconstruction model of lateral IL1 (yellow-tan) and IL2 (green) cilia indicating TZs (purple) and the long striated IL1 rootlet (white). Labels indicate approximate positions of cross-sections shown in B–D. (B–D) Cross-section TEM images of IL1 and IL2 at the distal segment which has few, disorganized sMTs (B), IL1 (Cii) and IL2 (Ci) TZs which include portions of the IL1 rootlet-like material (arrows). Scalebar: 100 nm. See Figure 13—figure supplement 1 for additional ultrastructural features present in IL1 and IL2 cilia.
Figure 13—figure supplement 1. Structural features associated with lateral IL1 and IL2 cilia.
(A) 3D reconstructed models of lateral IL1 and IL2 cilia and processes of associated support cells. TZs and CeAJs are indicated in purple and light blue, respectively. Approximate locations of cross-sections shown in B are indicated. (Bi–v) Shown are the IL2 plug (Bi; arrow), electron-dense disk at the tip of IL1 and material at the tip of IL2 (Bii, arrow and arrowhead, respectively), rootlet and rootlet-like structures (Biii; arrowheads); IL1 CeAJ and invaginations into the IL2 PCMC (Biv; arrow and arrowhead, respectively), and an IL2 CeAJ (Bv; arrow). Note that both cilia extend beyond their glial surrounds to enter a narrow pore in the lip cuticle (Bi, Bii). Scale bars: 500 nm (A), 100 nm (B).