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. Author manuscript; available in PMC: 2014 Sep 20.
Published in final edited form as: Oncogene. 2013 Apr 8;33(12):1558–1569. doi: 10.1038/onc.2013.97

Figure 3.

Figure 3

TIP-1 regulates the spatiotemporal activation of Rho GTPases in migrating glioblastoma cells. (a) Quantitative measurements of Rho GTPases. The active and total Rac1/Cdc42/RhoA in T98G cells were analyzed as described in Materials and methods. Results represent 3 independent experiments. The bar graph shows the densitometric quantifications of the blot results. (* p<0.002). (b) Staining of actin (red) with phalloidin to visualize stress filament formation along the leading edge of migrating T98G cells. (Scale bar: 20 μm). (c) Intracellular localization of Rho GTPases determined with immunofluorescent staining of migrating T98G cells. Four hours after cell scratching, cells were fixed and stained with antibodies as indicated. Arrows indicate the colocalized proteins. Scale bar= 40 μm. The fluorescence intensity of each protein was measured with ImageJ, and percentages of individual proteins located at the leading edge were calculated. (*p<0.001).