Figure 6.
TIP-1 knockdown has minor impact on the beta-catenin-controlled gene transcription and cell proliferation in glioblastoma cells. (a) Microarray profiling of gene expression in D54 glioblastoma cells with or without TIP-1 knockdown. Expression levels of a panel of beta-catenin-regulated genes are presented in a heat map. TIP-1 (Tax1bp3) was included as a quality control. (b) Western blot and semi-quantification of c-MYC and Cyclin D1 protein levels in D54 glioblastoma cells with or without TIP-1 knockdown. Data were respectively normalized to the levels of actin. (c) In vitro cell proliferation rates were measured by microscopic cell counting every 24 hours. (d) Quantitative measurement of cell proliferation rate in vivo. Intracranial glioma xenografts were resected 3 weeks after the tumor implantation. Quantification of Ki67 positive cells was based on at least 7 microscopic fields on more than 5 sections for each group. Data distribution was shown as box plot. p>0.05, ANOVA.