Figure 1.

Expression profile of S. acidocaldarius lrs14 genes during both biofilm and planktonic growth. Total RNA isolated from S. acidocaldarius MW001 and MW001^pyrEF+ (reference strains) grown either as biofilms or as planktonic cultures were used for complementary DNA (cDNA) synthesis. qRT-PCR analysis was performed using specific primers for each Lrs14-encoding ORF (shown underneath the plot). Relative transcript expression levels of each gene were normalized to the internal control gene secY. The values reflect the fold change in gene expression compared with cDNA prepared from exponential grown planktonic references strains cells, which is designated as baseline. The means and standard deviations of three biological replicates are shown.