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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Neuropharmacology. 2014 Jan 24;79:668–678. doi: 10.1016/j.neuropharm.2014.01.023

Figure 5. Degradation of Aβ40 within endosomal/lysosomal pathaway of bEnd3 and hCMEC/D3 cells.

Figure 5

Co-localization of intracellular Aβ40-HiLyte Fluor and LysoTracker Red in endosomal/lysosomal pathway of brain endothelial cells (bEnd3 and hCMEC/D3) analyzed using confocal immunoFluorescence microscope. bEnd3 cells and hCMEC/D3 were incubated with 1 nM of Aβ40-HiLyte Fluor for 15 min or 12 h; respectively. DAPI stains nuclei. Lysosomes were labeled with LysoTracker Red for 45 min before the end of study. The yellow color in the merged image represent co-localization of green (Aβ40-HiLyte Fluor) and red (LysoTracker Red) fluorescence.