Figure 8. Transgenic plants (OE and Ri) of OsPCF6 and OsTCP21 confer cold tolerance in rice.

(A) The survival rates of WT, OsPCF6 and OsTCP21 transgenic seedlings under normal condition and cold stress. (B) The proline contents of WT, OsPCF6 and OsTCP21 transgenic seedlings under normal condition and cold stress. Wild-type and transgenic rice seedlings of 2-week-old were exposed to 4°C for 3 days and then collected for determination of proline. (C) H₂O₂ accumulation in WT, OsPCF6 and OsTCP21 transgenic plants under cold stress. The 2-week-old seedling were exposed to 4°C for 3 d, and then seedling roots were cut off and directly incubated in the DAB dye solution for 8 h for H₂O₂ analysis. Left: WT, OsPCF6 and OsTCP21 transgenic plants without stress treatment; right: wild-type and transgenic plants, after treatment and staining. OE is dark brown; WT and Ri lines have the lightest color.