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. 2014 Mar 25;9(3):e91970. doi: 10.1371/journal.pone.0091970

Figure 1. Edelfosine impact on T cell viability.

Figure 1

(A) Gating strategy to determine frequencies of annexin V+ and/or PI+ CD4+ as well as CD8+ T cells. Dot plots for annexin V and PI gating of approaches in absence of edelfosine, with 10 µg/ml edelfosine, or 33.3 µg/ml edelfosine. (B) With regard to CD4+ T cells just 10 µg/ml edelfosine led to a decrease in annexin V−PI− frequencies accompanied by a remarkable increase in annexin V+PI+CD4+ T cell frequencies. In the case of CD8+ T cells a marked decrease in annexin V−PI− cells was only observed with 33.3 µg/ml edelfosine which resulted in comparable frequencies of annexin V+PI− as well as annexin V+PI+ cells. PBMCs of a donor were seeded in triplicates and pooled before analysis.