Table 2. Clustering of up- or downregulated genes to determine biological pathways affected by edelfosine in human CD4⁺ T cells after gene expression analysis.

A Setting: unstimultated + 10 µg/ml edelfosine vs. unstimulated
Up	Gene Examples	P-value
Apoptosis	JUN, RHOB, KRAS	1.8×10−2
Programmed cell death	JUN, RHOB, KRAS	1.9×10−2
Learning	JUN, KRAS	2.2×10−2
Down	Gene Examples	P-value
Immune response	CD74, CD79A, IGJ, IRF8, CCL22	3.9×10−14
Antigen processing and presentation of peptide or polysaccharide antigen via MHC class II	CD74, IFI30, HLA-DMA, HLA-DPA1	2.2×10−11
Antigen processing and presentation	CD74, IFI30, HLA-DMB, HLA-DRA	1.3×10−8

(A) The incubation of unstimulated cells with 10 µg/ml edelfosine resulted in the upregulation of apoptosis- and cell death-associated genes. Genes involved in immune response and antigen processing and presentation were downregulated. (B) In the case of stimulated cells which were cultured in presence of 3.3 µg/ml edelfosine the downmodulation of cell cycle progression-related genes was found. Additionally, edelfosine resulted in the upregulation of genes assigned to immune response- and virus response-pathways characterized by type I IFN-regulated genes.