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. 2014 Mar 25;9(3):e92381. doi: 10.1371/journal.pone.0092381

Figure 7. CS-E expression in EB cultures and proliferation.

Figure 7

(A) The CS-E-specific antibody GD3G7 detected widespread expression of CS-E moieties (no treatment). In the absence of primary antibody (no primary Ab), or after treatment with ChABC (ChABC treatment), no specific signal was observed. (Scale bar = 100 micrometer). (B) Quantification of proliferation by EdU incorporation in EB cultures, in the presence and absence of treatments with ChABC, C4S and CS-E. At either d6 or d12 of EB cultures, elimination of endogenous CS by treatment with ChABC, or treatment with exogenous C4S or CS-E did not significantly alter the percentage of EdU-positive cells when compared to untreated controls.