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. 2014 Mar 25;9(3):e92181. doi: 10.1371/journal.pone.0092181

Figure 3. Expression analysis of the different mutants.

Figure 3

A: Representative western blot showing expression levels of wild type CYP21 and the different mutagenized constructions assayed. B: Densitometric quantification of CYP21A2 expression. Protein levels of the different CYP21A2 constructs were expressed as the ratio between CYP21A2 and β-actin, considering β-galactosidase activity as the control for transfection efficiency. Bars represent the mean ± S.E.M of 2 different extracts assayed 2 times each.